TRPC4AP Polyclonal antibody
TRPC4AP Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HuH-7 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29310-1-AP targets TRPC4AP in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Tested Reactivity | human |
Immunogen | TRPC4AP fusion protein Ag29860 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transient receptor potential cation channel, subfamily C, member 4 associated protein |
Synonyms | Trpc4-associated protein, Trp4-associated protein, TNF-receptor ubiquitous scaffolding/signaling protein, Short transient receptor potential channel 4-associated protein, PPP1R158 |
Calculated Molecular Weight | 797 aa, 91 kDa |
Observed Molecular Weight | 85-90 kDa |
GenBank Accession Number | BC013144 |
Gene Symbol | TRPC4AP |
Gene ID (NCBI) | 26133 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8TEL6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for TRPC4AP antibody 29310-1-AP | Download protocol |
IHC protocol for TRPC4AP antibody 29310-1-AP | Download protocol |
IP protocol for TRPC4AP antibody 29310-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |