验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells, HuH-7 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human ovary cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29310-1-AP targets TRPC4AP in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
经测试反应性 | human |
免疫原 | TRPC4AP fusion protein Ag29860 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | transient receptor potential cation channel, subfamily C, member 4 associated protein |
别名 | Trpc4-associated protein, Trp4-associated protein, TNF-receptor ubiquitous scaffolding/signaling protein, Short transient receptor potential channel 4-associated protein, PPP1R158 |
计算分子量 | 797 aa, 91 kDa |
观测分子量 | 85-90 kDa |
GenBank蛋白编号 | BC013144 |
基因名称 | TRPC4AP |
Gene ID (NCBI) | 26133 |
RRID | AB_3086113 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TEL6 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.
实验方案
Product Specific Protocols | |
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WB protocol for TRPC4AP antibody 29310-1-AP | Download protocol |
IHC protocol for TRPC4AP antibody 29310-1-AP | Download protocol |
IP protocol for TRPC4AP antibody 29310-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |