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验证数据展示

  • WB analysis of human brain using 66138-1-Ig

    WB analysis of human brain using 66138-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66138-1-Ig (TRPC4AP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 66138-1-Ig

    IHC staining of human liver using 66138-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver using 66138-1-Ig(TRPC4AP antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 66138-1-Ig

    IHC staining of human breast cancer using 66138-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66138-1-Ig (TRPC4AP Antibody) at dilution of 1:50.

TRPC4AP Monoclonal antibody

TRPC4AP Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66138-1-Ig

Print datasheet

CloneNo. 2C10B1

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, human heart tissue
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66138-1-Ig targets TRPC4AP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen TRPC4AP fusion protein Ag18772 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily C, member 4 associated protein
Synonyms C20orf188, Protein TAP1, Protein TRUSS, Trp4 associated protein, Trpc4 associated protein, TRPC4AP, TRRP4AP, TRUSS
Calculated Molecular Weight 797 aa, 91 kDa
Observed Molecular Weight 82 kDa
GenBank Accession NumberBC013144
Gene Symbol TRPC4AP
Gene ID (NCBI) 26133
RRIDAB_2881537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8TEL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPC4AP, also named as C20orf188 and TRRP4AP, is a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. TRPC4AP participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. The calculated molecular weight of TRPC4AP is 91 kDa. Western blot analysis detected a protein of about 82 kDa expressed in several mouse cell lines and in a human promonocytic cell line.

实验方案

Product Specific Protocols
WB protocol for TRPC4AP antibody 66138-1-IgDownload protocol
IHC protocol for TRPC4AP antibody 66138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols