TRPC5 Polyclonal antibody

TRPC5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25890-1-AP

Print datasheet

Synonyms

TRP5, TRP 5, Transient receptor protein 5, hTRP-5, hTRP5



经过测试的应用

Positive WB detected inHL-60 cells, MCF-7 cells
Positive IHC detected inhuman brain tissue, human cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25890-1-AP targets TRPC5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen TRPC5 fusion protein Ag23171 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily C, member 5
Synonyms TRP5, TRP 5, Transient receptor protein 5, hTRP-5, hTRP5
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC137271
Gene Symbol TRPC5
Gene ID (NCBI) 7224
RRIDAB_2880285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UL62
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Short transient receptor potential channel 5(TRPC5), which belongs to TRPC5 sub-subfamily of transient receptor (TC 1.A.4) family, thought to form a receptor-activated non-selective calcium permeant cation channel, and probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. TRPC5 has also been shown to be calcium-selective and may also be activated by intracellular calcium store depletion.

实验方案

Product Specific Protocols
WB protocol for TRPC5 antibody 25890-1-APDownload protocol
IHC protocol for TRPC5 antibody 25890-1-APDownload protocol
IF protocol for TRPC5 antibody 25890-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

Endothelial cell Orai1 is essential for endothelium-dependent contraction of mouse carotid arteries in normotensive and hypertensive mice

Authors - Xiao Li
humanWB

Cancer Lett

TRPC3 promotes tumorigenesis of gastric cancer via the CNB2/GSK3β/NFATc2 signaling pathway.

Authors - Da-Cen Lin
humanWB

Elife

Structural basis for human TRPC5 channel inhibition by two distinct inhibitors.

Authors - Kangcheng Song
mouseWB,IF

J Neuroinflammation

Upregulation of TRPC5 in hippocampal excitatory synapses improves memory impairment associated with neuroinflammation in microglia knockout IL-10 mice.

Authors - Shiji Huo
humanWB

J Mol Cell Cardiol

TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes.

Authors - Zhichao Li
mouseIF

Biochem Pharmacol

Endothelial cell transient receptor potential channel C5 (TRPC5) is essential for endothelium-dependent contraction in mouse carotid arteries.

Authors - Cai Liang