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TRPM2 Polyclonal antibody

TRPM2 Polyclonal Antibody for IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 21842-1-AP

Print datasheet

Synonyms

LTrpC 2, KNP3, KNP 3, Estrogen-responsive element-associated gene 1 protein, EREG1

验证数据展示

  • IHC staining of mouse brain using 21842-1-AP

    IHC staining of mouse brain using 21842-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21842-1-AP (TRPM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21842-1-AP

    IHC staining of mouse brain using 21842-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21842-1-AP (TRPM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 21842-1-AP

    IF Staining of mouse brain using 21842-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRPM2 antibody (21842-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 21842-1-AP

    IF Staining of mouse brain using 21842-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using TRPM2 antibody (21842-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21842-1-AP targets TRPM2 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TRPM2 fusion protein Ag16522 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily M, member 2
Synonyms LTrpC 2, KNP3, KNP 3, Estrogen-responsive element-associated gene 1 protein, EREG1
Calculated Molecular Weight 1553 aa, 177 kDa
GenBank Accession NumberBC112342
Gene Symbol TRPM2
Gene ID (NCBI) 7226
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94759
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TRPM2 antibody 21842-1-APDownload protocol
IF protocol for TRPM2 antibody 21842-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols