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验证数据展示

  • WB analysis using 12724-1-AP

    WB analysis using 12724-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12724-1-AP (TRPM3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

TRPM3 Polyclonal antibody

TRPM3 Polyclonal Antibody for WB, ELISA
Cat No. 12724-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

GON 2, LTRPC3, MLSN2, TRPM3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12724-1-AP targets TRPM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TRPM3 fusion protein Ag3421 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 transient receptor potential cation channel, subfamily M, member 3
别名 GON 2, LTRPC3, MLSN2, TRPM3
计算分子量 1732 aa, 197 kDa
观测分子量160-210 kDa
GenBank蛋白编号BC022454
基因名称 TRPM3
Gene ID (NCBI) 80036
RRIDAB_2935431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCF6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transient receptor potential melastatin-3 (TRPM3) channel belongs to the family of transient receptor potential (TRP) cation channels that are expressed in a variety of tissues and cell types, including kidney, brain, dorsal root ganglia, cardiomyocytes and pancreatic beta-cells. TRPM3 channels can be activated by the neurosteroid pregnenolone sulfate, and they are regulated by phosphoinositides, and perhaps by calmodulin. Stimulation of TRPM3 channels is connected with heat sensation by somatosensory neurons, insulin secretion by pancreatic beta-cells, regulation of neurotransmitter release, iris constriction, and tumor promotion. The calculated MW of TRPM3 is 197 kDa, 12724-1-AP can detect a band around 160 kDa, which likely corresponds to different splice variant. (PMID: 28720517,21198543)

实验方案

Product Specific Protocols
WB protocol for TRPM3 antibody 12724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols