验证数据展示
经过测试的应用
| Positive WB detected in | 37°C incubated HEK-293 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21985-1-AP targets TRPM4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | TRPM4 fusion protein Ag17132 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transient receptor potential cation channel, subfamily M, member 4 |
| 别名 | hTRPM4, LTrpC 4, LTrpC4, Melastatin 4, TRPM4, TRPM4B |
| 计算分子量 | 1214 aa, 134 kDa |
| 观测分子量 | 130~150 kDa |
| GenBank蛋白编号 | BC132727 |
| 基因名称 | TRPM4 |
| Gene ID (NCBI) | 54795 |
| RRID | AB_2935456 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TD43 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPM4 is a Calcium-activated nonselective cation channel. It is activated by increase in intracellular Ca2+, mediates transport of monovalent cations (Na+ > K+ > Cs+ > Li+), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. TRPM4 participates in T-cell activation by modulating Ca2+ oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. It's also involved in myogenic constriction of cerebral arteries.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPM4 antibody 21985-1-AP | Download protocol |
| IHC protocol for TRPM4 antibody 21985-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Comput Struct Biotechnol J Identification of a 24-gene panel and a novel marker of PODXL2 essential for the pathological diagnosis of early prostate cancer | ||
Tissue Cell Transient receptor potential channel TRPM4 favors oxidized low-density lipoprotein-induced coronary endothelial cell dysfunction via a mechanism involving ferroptosis
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