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验证数据展示

  • WB analysis using 21938-1-AP

    WB analysis using 21938-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21938-1-AP (TRPS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 21938-1-AP

    WB analysis of HEK-293 using 21938-1-AP

    WB result of TRPS1 antibody (21938-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRPS1 transfected HEK-293 cells.

  • IP experiment of mouse lung using 21938-1-AP

    IP experiment of mouse lung using 21938-1-AP

    IP result of anti-TRPS1 (IP:21938-1-AP, 4ug; Detection:21938-1-AP 1:1000) with mouse lung tissue lysate 3600ug.

  • IF Staining of mouse skin using 21938-1-AP

    IF Staining of mouse skin using 21938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse skin using 21938-1-AP

    IF Staining of mouse skin using 21938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 21938-1-AP

    IF Staining of MCF-7 using 21938-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TRPS1 antibody (21938-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), (CL488-Phalloidin, green).

  • Featured Product
  • KD/KO验证

TRPS1 Polyclonal antibody

TRPS1 Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA
Cat No. 21938-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IF-P, IP, ELISA and More (1)

别名

GC79, LGCR, Tricho-rhino-phalangeal syndrome type I protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse testis tissue
Positive IP detected inmouse lung tissue
Positive IF-P detected inmouse skin tissue
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 2 publications below

产品信息

21938-1-AP targets TRPS1 in WB, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TRPS1 fusion protein Ag16583 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 trichorhinophalangeal syndrome I
别名 GC79, LGCR, Tricho-rhino-phalangeal syndrome type I protein
计算分子量 1294 aa, 143 kDa
观测分子量 150 kDa
GenBank蛋白编号BC125020
基因名称 TRPS1
Gene ID (NCBI) 7227
RRIDAB_11232233
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains

实验方案

Product Specific Protocols
WB protocol for TRPS1 antibody 21938-1-APDownload protocol
IF protocol for TRPS1 antibody 21938-1-APDownload protocol
IP protocol for TRPS1 antibody 21938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Biochim Biophys Acta Mol Basis Dis

UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation

Authors - Yushan Zhang
View Article
mouseIF

Acta Biomater

Shape-Fixing Hydrogel Promotes Scarless Healing of Wounds Under Tension

Authors - Danlan Fu
View Article
humanIF

Clin Transl Oncol

Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer

Authors - Dapeng Zhao
View Article