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TRPS1 Polyclonal antibody
TRPS1 Polyclonal Antibody for WB, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF-P, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, mouse kidney tissue, mouse lung tissue, mouse testis tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IF-P detected in | mouse skin tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 2 publications below |
产品信息
21938-1-AP targets TRPS1 in WB, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IF-P, IP, ELISA Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | TRPS1 fusion protein Ag16583 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | trichorhinophalangeal syndrome I |
| Synonyms | GC79, LGCR, TRPS1, Zinc finger protein GC79 |
| Calculated Molecular Weight | 1294 aa, 143 kDa |
| Observed Molecular Weight | 150 kDa |
| GenBank Accession Number | BC125020 |
| Gene Symbol | TRPS1 |
| Gene ID (NCBI) | 7227 |
| RRID | AB_11232233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHF7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPS1 is a zinc finger transcriptional repressor involved in the regulation of chondrocyte and perichondrium development, containing a GATA-type zinc finger through which it binds to DNA. with nine zinc-finger domains and two C-terminal Ikaros-like zinc fingers. Its reperssible function was dependent on the integrity of the Trps1 GATA-type zinc-finger domain and also required the C-terminal 119 amino acids of the protein, which harbor the two Ikaros-like zinc-finger domains
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPS1 antibody 21938-1-AP | Download protocol |
| IF protocol for TRPS1 antibody 21938-1-AP | Download protocol |
| IP protocol for TRPS1 antibody 21938-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis UVB-induced TRPS1 regulates MITF transcription activity to promote skin pigmentation | ||
Clin Transl Oncol Endoplasmic reticulum stress regulates apoptosis and chemotherapeutic via enhancing TNFRSF10B recycling to the cell membrane in triple-negative breast cancer |
