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验证数据展示

  • IHC staining of human breast cancer using 29971-1-AP

    IHC staining of human breast cancer using 29971-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 29971-1-AP (TRPV3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRPV3 antibody included

TRPV3 Polyclonal antibody

TRPV3 Polyclonal Antibody for IHC, ELISA
Cat No. 29971-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, ELISA

Synonyms

TRPV3, Vanilloid receptor like 3, VRL 3, VRL3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRPV3 Ready-To-Use IHC Kit. TRPV3 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29971-1-AP targets TRPV3 in IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Human
Immunogen TRPV3 fusion protein Ag32437 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily V, member 3
Synonyms TRPV3, Vanilloid receptor like 3, VRL 3, VRL3
Calculated Molecular Weight91kd
GenBank Accession NumberNM_001258205
Gene Symbol TRPV3
Gene ID (NCBI) 162514
RRIDAB_3086200
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NET8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transient receptor potential cation channel, subfamily V, member 3, also known as TRPV3, is a human gene encoding the protein of the same name. The TRPV3 protein belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of human sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. The TRPV3 channel has wide tissue expression that is especially high in the skin (keratinocytes) but also in the brain. It functions as a molecular sensor for innocuous warm temperatures.( PMID 12016205)

实验方案

Product Specific Protocols
IHC protocol for TRPV3 antibody 29971-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols