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  • KD/KO Validated

TRPV4 Polyclonal antibody

TRPV4 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 20987-1-AP

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Synonyms

Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC



经过测试的应用

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KOSee 2 publications below
IHCSee 6 publications below

产品信息

20987-1-AP targets TRPV4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transient receptor potential cation channel, subfamily V, member 4
Synonyms Osm 9 like TRP channel 4, OTRPC4, TRP12, TRPV4, VR OAC, VRL 2, VRL2, VROAC
Calculated Molecular Weight 98 kDa
GenBank Accession NumberNM_021625
Gene Symbol TRPV4
Gene ID (NCBI) 59341
RRIDAB_10926386
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HBA0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRPV4, also named as VRL2 and VROAC, belongs to the transient receptor family and TrpV subfamily. It is a non-selective calcium permeant cation channel protein which involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. It is activated by low pH, citrate and phorbol esters. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Defects in TRPV4 are the cause of brachyolmia type 3 (BRAC3), spondylometaphyseal dysplasia Kozlowski type (SMDK) and metatropic dysplasia (MTD). Multiple transcript variants encoding different isoforms have been found.

实验方案

Product Specific Protocols
IHC protocol for TRPV4 antibody 20987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Autophagy

Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4.

Authors - Tengfei Huang
  • KD Validated
human

Cell Death Dis

Inhibition of TRPV4 remodels single cell polarity and suppresses the metastasis of hepatocellular carcinoma

Authors - Jian Liu
mouseIHC

Environ Pollut

Exposure to both formaldehyde and high relative humidity exacerbates allergic asthma by activating the TRPV4-p38 MAPK pathway in Balb/c mice.

Authors - Jiufei Duan
mouse

Sci Rep

Phosphodiesterase 3B (PDE3B) regulates NLRP3 inflammasome in adipose tissue.

Authors - Faiyaz Ahmad
mouse

Neuropharmacology

TRPV4 inhibitor HC067047 produces antidepressant-like effect in LPS-induced depression mouse model.

Authors - Wei Li

Cell Mol Bioeng

The mechanosensitive ion channel TRPV4 is a regulator of lung development and pulmonary vasculature stabilization.

Authors - Joshua T Morgan