验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse kidney tissue, PC-3 cells |
| Positive IP detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| IF | See 5 publications below |
产品信息
13411-1-AP targets TRPV6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | TRPV6 fusion protein Ag4060 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transient receptor potential cation channel, subfamily V, member 6 |
| 别名 | CATL, CaT1, CaT like, CaT L, Calcium transport protein 1 |
| 计算分子量 | 725 aa, 83 kDa |
| 观测分子量 | 83 kDa, 70 kDa |
| GenBank蛋白编号 | BC034814 |
| 基因名称 | TRPV6 |
| Gene ID (NCBI) | 55503 |
| RRID | AB_2272390 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H1D0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRPV6, also named as CAT1 or ECaC2, is a member of the transient receptor potential (TRP) family of membrane proteins. Unlike most TRP channels, TRPV6 and its closest relative, TRPV5, are calcium-selective channels. TRPV6 is highly expressed in the proximal intestine, placenta and exocrine tissues (PMID: 12869611). It is probably involved in calcium absorption in various tissues, including calcium reabsorption in kidney. TRPV6 is overexpressed in some cancers and exhibits oncogenic potential.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRPV6 antibody 13411-1-AP | Download protocol |
| IF protocol for TRPV6 antibody 13411-1-AP | Download protocol |
| IP protocol for TRPV6 antibody 13411-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Mol Cell Cardiol TRPV6 protects ER stress-induced apoptosis via ATF6α-TRPV6-JNK pathway in human embryonic stem cell-derived cardiomyocytes. | ||
Front Pharmacol Aqueous Extract of Mori Folium Exerts Bone Protective Effect Through Regulation of Calcium and Redox Homeostasis via PTH/VDR/CaBP and AGEs/RAGE/Nox4/NF-κB Signaling in Diabetic Rats. | ||
J Gastroenterol Hepatol Vitamin D receptor is overexpressed in the duodenum of patients with irritable bowel syndrome. | ||
Mol Reprod Dev Proteomic analysis of seminal extracellular vesicle proteins involved in asthenozoospermia by iTRAQ. |
