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TRUB2 Polyclonal antibody

TRUB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19891-1-AP

Print datasheet

Synonyms

CLONE24922, RP11 339B21.1, TRUB2

验证数据展示

  • WB analysis of HeLa using 19891-1-AP

    WB analysis of HeLa using 19891-1-AP

    WB result of TRUB2 antibody (19891-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRUB2 transfected HeLa cells.

  • WB analysis using 19891-1-AP

    WB analysis using 19891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 19891-1-AP

    IHC staining of human colon cancer using 19891-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRUB2 antibody included
  • IHC staining of human colon cancer using 19891-1-AP

    IHC staining of human colon cancer using 19891-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRUB2 antibody included

经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRUB2 Ready-To-Use IHC Kit. TRUB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19891-1-AP targets TRUB2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TRUB2 fusion protein Ag13726 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TruB pseudouridine (psi) synthase homolog 2 (E. coli)
Synonyms CLONE24922, RP11 339B21.1, TRUB2
Calculated Molecular Weight 331 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC001457
Gene Symbol TRUB2
Gene ID (NCBI) 26995
RRIDAB_10640900
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRUB2 antibody 19891-1-APDownload protocol
IHC protocol for TRUB2 antibody 19891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
View Article
humanWB

Nat Cell Biol

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression

Authors - Nicholas J Kramer
View Article