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验证数据展示

  • WB analysis of HeLa using 19891-1-AP

    WB analysis of HeLa using 19891-1-AP

    WB result of TRUB2 antibody (19891-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRUB2 transfected HeLa cells.

  • WB analysis using 19891-1-AP

    WB analysis using 19891-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 19891-1-AP

    IHC staining of human colon cancer using 19891-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRUB2 antibody included
  • IHC staining of human colon cancer using 19891-1-AP

    IHC staining of human colon cancer using 19891-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19891-1-AP (TRUB2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TRUB2 antibody included
  • Featured Product
  • KD/KO验证

TRUB2 Polyclonal antibody

TRUB2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 19891-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CLONE24922, RP11 339B21.1, TRUB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, HeLa cells, HepG2 cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TRUB2 Ready-To-Use IHC Kit. TRUB2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19891-1-AP targets TRUB2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 TRUB2 fusion protein Ag13726 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TruB pseudouridine (psi) synthase homolog 2 (E. coli)
别名 CLONE24922, RP11 339B21.1, TRUB2
计算分子量 331 aa, 37 kDa
观测分子量 37 kDa
GenBank蛋白编号BC001457
基因名称 TRUB2
Gene ID (NCBI) 26995
RRIDAB_10640900
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95900
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TRUB2 antibody 19891-1-APDownload protocol
IHC protocol for TRUB2 antibody 19891-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO Rep

A pseudouridine synthase module is essential for mitochondrial protein synthesis and cell viability.

Authors - Hana Antonicka
View Article
humanWB

Nat Cell Biol

Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression

Authors - Nicholas J Kramer
View Article