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Hamartin/TSC1 Polyclonal antibody

Hamartin/TSC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 29906-1-AP

Print datasheet

Synonyms

Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein

验证数据展示

  • WB analysis using 29906-1-AP

    WB analysis using 29906-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29906-1-AP (TSC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 29906-1-AP

    WB analysis of mouse kidney using 29906-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse kidney using 29906-1-AP

    IHC staining of mouse kidney using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC1 antibody included
  • IHC staining of mouse kidney using 29906-1-AP

    IHC staining of mouse kidney using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC1 antibody included
  • IHC staining of mouse skeletal muscle using 29906-1-AP

    IHC staining of mouse skeletal muscle using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC1 antibody included
  • IHC staining of mouse skeletal muscle using 29906-1-AP

    IHC staining of mouse skeletal muscle using 29906-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 29906-1-AP (Hamartin/TSC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSC1 antibody included

经过测试的应用

Positive WB detected inHeLa cells, mouse kidney tissue, MCF-7 cells
Positive IHC detected inmouse kidney tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TSC1 Ready-To-Use IHC Kit. TSC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29906-1-AP targets Hamartin/TSC1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen Hamartin/TSC1 fusion protein Ag31621 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tuberous sclerosis 1
Synonyms Hamartin, KIAA0243, LAM, TSC, TSC1, tuberous sclerosis 1, Tuberous sclerosis 1 protein
Calculated Molecular Weight130KD
Observed Molecular Weight140-145 kDa
GenBank Accession NumberNM_000368
Gene Symbol Hamartin/TSC1
Gene ID (NCBI) 7248
RRIDAB_2918352
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC1, also named as hamartin, interacts with TSC2 to form a protein complex that inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (PubMed: 12271141, PubMed: 28215400). It can recruit TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (PubMed: 16464865, PubMed: 29127155). The loss of TSC1/TSC2 in mouse neurons resulted in a block in oligodendrocyte hypomyelination in vivo (PubMed: 28183733).

实验方案

Product Specific Protocols
WB protocol for Hamartin/TSC1 antibody 29906-1-APDownload protocol
IHC protocol for Hamartin/TSC1 antibody 29906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Elife

A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression

Authors - Chaojun Yan
View Article
humanWB

Cell Signal

METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression

Authors - Mengmeng Zhang
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humanWB,IP,IHC,IF

Br J Cancer

CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitors

Authors - Xinlin Liu
View Article