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  • Featured Product
  • KD/KO Validated

TSC22D4 Polyclonal antibody

TSC22D4 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55017-1-AP

Print datasheet

Synonyms

THG 1, THG1, TILZ2, Tsc 22 like protein THG 1, TSC22 domain family protein 4, TSC22 domain family, member 4, TSC22D4

验证数据展示

  • WB analysis of HeLa using 55017-1-AP

    WB analysis of HeLa using 55017-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55017-1-AP (TSC22D4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 55017-1-AP

    IF Staining of HeLa using 55017-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 55017-1-AP (TSC22D4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55017-1-AP targets TSC22D4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TSC22 domain family, member 4
Synonyms THG 1, THG1, TILZ2, Tsc 22 like protein THG 1, TSC22 domain family protein 4, TSC22 domain family, member 4, TSC22D4
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41-45 kDa
GenBank Accession NumberNM_030935
Gene Symbol TSC22D4
Gene ID (NCBI) 81628
RRIDAB_10860903
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y3Q8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSC22D4, also named as TILZ2 and THG-1, is a transcriptional repressor. It is a leucine zipper-containing protein that is highly conserved during evolution. TSC22D4 is transcriptionally up-regulated by many different stimuli, including anti-cancer drugs and growth inhibitors. It plays a suppressive role in tumorigenesis. TSC22D4 interacts with TSC22D1 by forming heterodimers(PMID:10488076).This antibody is specific to TSC22D4.

实验方案

Product Specific Protocols
WB protocol for TSC22D4 antibody 55017-1-APDownload protocol
IF protocol for TSC22D4 antibody 55017-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

J Cell Physiol

The interplay between TGF-β-stimulated TSC22 domain family proteins regulates cell-cycle dynamics in medulloblastoma cells

Authors - Jessica Dragotto
  • KD Validated
View Article