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  • Featured Product
  • KD/KO Validated

TSEN2 Polyclonal antibody

TSEN2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13103-2-AP

Print datasheet

Synonyms

tRNA-intron endonuclease Sen2, SEN2L, SEN2, PCH2B, HsSen2

验证数据展示

  • WB analysis of mouse colon using 13103-2-AP

    WB analysis of mouse colon using 13103-2-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 13103-2-AP (TSEN2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13103-2-AP

    IF Staining of HeLa using 13103-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13103-2-AP (TSEN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse colon tissue, human brain tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13103-2-AP targets TSEN2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TSEN2 fusion protein Ag3742 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tRNA splicing endonuclease 2 homolog (S. cerevisiae)
Synonyms tRNA-intron endonuclease Sen2, SEN2L, SEN2, PCH2B, HsSen2
Calculated Molecular Weight 465 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC019582
Gene Symbol TSEN2
Gene ID (NCBI) 80746
RRIDAB_2272213
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSEN2, also named as SEN2, belongs to the tRNA-intron endonuclease family. TSEN2 is a metal ion independent nuclease that cleave the 5' splice site (PMID: 32476018). TSEN2 has 5 isoforms with the molecular mass of 45-53 kDa. TSEN2 is involved in the formation of a human endonuclease complex whose function is the removal of introns from pre-tRNA (PMID: 16595010).

实验方案

Product Specific Protocols
WB protocol for TSEN2 antibody 13103-2-APDownload protocol
IF protocol for TSEN2 antibody 13103-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

CLP1 Founder Mutation Links tRNA Splicing and Maturation to Cerebellar Development and Neurodegeneration.

Authors - Ashleigh E Schaffer
View Article
humanWB

Am J Hum Genet

Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly.

Authors - Martin W Breuss
View Article
humanWB

Genetics

An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching

Authors - Jennifer E Hurtig
  • KD Validated
View Article