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  • Featured Product
  • KD/KO Validated

TSFM Polyclonal antibody

TSFM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11701-1-AP

Print datasheet

Synonyms

EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3

验证数据展示

  • WB analysis of HeLa using 11701-1-AP

    WB analysis of HeLa using 11701-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11701-1-AP (TSFM Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11701-1-AP

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon cancer using 11701-1-AP

    IHC staining of human colon cancer using 11701-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11701-1-AP (TSFM antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HeLa using 11701-1-AP

    IF Staining of HeLa using 11701-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 11701-1-AP (TSFM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, PC-3 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human kidney tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11701-1-AP targets TSFM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen TSFM fusion protein Ag2308 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Ts translation elongation factor, mitochondrial
Synonyms EF-TsMt, EF-Ts, EF Tsmt, EF TS, COXPD3
Calculated Molecular Weight 35 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC022862
Gene Symbol TSFM
Gene ID (NCBI) 10102
RRIDAB_2207953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP43897
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSFM antibody 11701-1-APDownload protocol
IHC protocol for TSFM antibody 11701-1-APDownload protocol
IF protocol for TSFM antibody 11701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Biol Rep

Short-term regulation of TSFM level does not alter amyloidogenesis and mitochondrial function in type-specific cells

Authors - Xiao-Yun Li
  • KD Validated
View Article