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验证数据展示

  • WB analysis using 84334-4-RR

    WB analysis using 84334-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84334-4-RR (TSG101 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 84334-4-RR

    IHC staining of human colon cancer using 84334-4-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84334-4-RR (TSG101 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSG101 antibody included
  • Affinity and Kinetic Characterization of 84334-4-RR

    Affinity and Kinetic Characterization of 84334-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84334-4-RR against Human TSG101 were performed. The affinity constant is below 1 pM.

TSG101 Recombinant antibody

TSG101 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84334-4-RR

产品说明书

CloneNo. 241657A1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, JurKat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TSG101 Ready-To-Use IHC Kit. TSG101 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84334-4-RR targets TSG101 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TSG101 fusion protein Ag28569 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 tumor susceptibility gene 101
别名 VPS 23, TSG10, TSG 101, TSG 10, ESCRT-I complex subunit TSG101
计算分子量 44 kDa
观测分子量44 kDa
GenBank蛋白编号BC002487
基因名称 TSG101
Gene ID (NCBI) 7251
RRIDAB_3671874
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ99816
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSG101(Tumor susceptibility gene 101 protein) is essential for endosomal sorting, membrane receptor degradation and the final stages of cytokinesis. It plays a crucial role for cell proliferation and cell survival. TSG101 has been identified as a candidate tumor suppressor gene and belongs to the ubiquitin-conjugating enzyme family. TSG101 is a marker for exosome. This protein has 2 isoforms produced by alternative splicing with the molecular mass of 44 and 32 kDa.

实验方案

Product Specific Protocols
WB protocol for TSG101 antibody 84334-4-RRDownload protocol
IHC protocol for TSG101 antibody 84334-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols