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TSGA10 Polyclonal antibody

TSGA10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12593-1-AP

Print datasheet

Synonyms

CEP4L, testis specific, 10, TSGA10

验证数据展示

  • WB analysis of rat testis using 12593-1-AP

    WB analysis of rat testis using 12593-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 12593-1-AP (TSGA10 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 12593-1-AP

    IHC staining of human testis using 12593-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 12593-1-AP

    IHC staining of human testis using 12593-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12593-1-AP (TSGA10 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inrat testis tissue, mouse testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12593-1-AP targets TSGA10 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TSGA10 fusion protein Ag3311 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name testis specific, 10
Synonyms CEP4L, testis specific, 10, TSGA10
Calculated Molecular Weight 698 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC028366
Gene Symbol TSGA10
Gene ID (NCBI) 80705
RRIDAB_2240967
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BZW7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSGA10 antibody 12593-1-APDownload protocol
IHC protocol for TSGA10 antibody 12593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Front Cell Dev Biol

miR-129-5p Inhibits Bone Formation Through TCF4.

Authors - Chong Yin
View Article
humanWB

Front Cell Dev Biol

Pathogenic Variants in ACTRT1 Cause Acephalic Spermatozoa Syndrome.

Authors - Yanwei Sha
View Article
human,mouseWB，IHC

J Cell Mol Med

MACF1 alleviates aging-related osteoporosis via HES1.

Authors - Chong Yin
View Article
humanWB,IF

Clin Genet

TSGA10 is a novel candidate gene associated with acephalic spermatozoa.

Authors - Yan-Wei Sha
View Article
humanWB

J Assist Reprod Genet

Pathogenesis of acephalic spermatozoa syndrome caused by splicing mutation and de novo deletion in TSGA10.

Authors - Mingfei Xiang
View Article
humanWB,IF

Basic Clin Androl

A novel homozygous TSGA10 missense variant causes acephalic spermatozoa syndrome in a Pakistani family

Authors - Khalid Khan
View Article