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TSKU Polyclonal antibody

TSKU Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12370-1-AP

Print datasheet

Synonyms

E2 induced gene 4 protein, E2IG4, LRRC54, TSK, TSKU, Tsukushi, tsukushin

验证数据展示

  • WB analysis of mouse liver using 12370-1-AP

    WB analysis of mouse liver using 12370-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 12370-1-AP (TSKU Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12370-1-AP

    IHC staining of human colon cancer using 12370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12370-1-AP

    IHC staining of human colon cancer using 12370-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12370-1-AP (TSKU Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, HepG2 cells, mouse colon tissue, mouse pancreas tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12370-1-AP targets TSKU in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TSKU fusion protein Ag3054 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tsukushin
Synonyms E2 induced gene 4 protein, E2IG4, LRRC54, TSK, TSKU, Tsukushi, tsukushin
Calculated Molecular Weight 353 aa, 38 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC020975
Gene Symbol TSKU
Gene ID (NCBI) 25987
RRIDAB_2877851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSK, also known as Tsukushi, is an atypical member of the small leucine-rich proteoglycan (SLRP) family of extracellular matrix proteins (PMID: 16884925). The human TSK cDNA encodes a 16 amino acid (aa) signal sequence and a 337 aa secreted protein that contains nine LRR, which has a calculated molecular mass of 38kDa and an apparent molecular mass of 40-50kDa cause of the N-glycosylation. In chick embryos, C-TSK is a unique BMP inhibitor that forms a ternary complex with BMP and chordin and is involved in the regulation of primitive streak and node formation. C-TSK migrates as a band of 47 kDa in SDS-PAGE and N-glycosidase F treatment reduces the size to 41 kDa (PMID: 15363410).

实验方案

Product Specific Protocols
WB protocol for TSKU antibody 12370-1-APDownload protocol
IHC protocol for TSKU antibody 12370-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols