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TSN Polyclonal antibody

TSN Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 55426-1-AP

Print datasheet

Synonyms

REHF 1, translin, TRSLN, TSN

验证数据展示

  • WB analysis of Jurkat using 55426-1-AP

    WB analysis of Jurkat using 55426-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55426-1-AP (TSN Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 55426-1-AP

    IP experiment of MCF-7 using 55426-1-AP

    IP result of anti-TSN (IP:55426-1-AP, 3ug; Detection:55426-1-AP 1:300) with MCF-7 cells lysate 1600ug.

  • IHC staining of mouse testis using 55426-1-AP

    IHC staining of mouse testis using 55426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSN antibody included
  • IHC staining of mouse brain using 55426-1-AP

    IHC staining of mouse brain using 55426-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55426-1-AP (TSN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TSN antibody included

经过测试的应用

Positive WB detected inJurkat cells, HeLa cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TSN Ready-To-Use IHC Kit. TSN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55426-1-AP targets TSN in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name translin
Synonyms REHF 1, translin, TRSLN, TSN
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberNM_004622
Gene Symbol TSN
Gene ID (NCBI) 7247
RRIDAB_2881326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15631
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSN, also named as Translin or Component 3 of promoter of RISC, is a 228 amino acid protein, which belongs to the translin family. TSN as a DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translations, mostly is involved in immunoglobulin T-cell receptor gene segments. TSN May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.

实验方案

Product Specific Protocols
WB protocol for TSN antibody 55426-1-APDownload protocol
IHC protocol for TSN antibody 55426-1-APDownload protocol
IP protocol for TSN antibody 55426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols