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  • 概述
  • 经过测试的应用
  • 产品信息
  • 实验方案

TSNAX Polyclonal antibody

TSNAX Polyclonal Antibody for IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11040-1-AP

Print datasheet

Synonyms

translin associated factor X, Translin associated protein X, TRAX, TSNAX

验证数据展示

  • IHC staining of human gliomas using 11040-1-AP

    IHC staining of human gliomas using 11040-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11040-1-AP (TSNAX antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of MCF-7 using 11040-1-AP

    IF Staining of MCF-7 using 11040-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TSNAX antibody 11040-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HeLa using 11040-1-AP

    IP experiment of HeLa using 11040-1-AP

    IP result of anti-TSNAX (IP:11040-1-AP, 4ug; Detection:11040-1-AP 1:300) with HeLa cells lysate 2400ug.

经过测试的应用

Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11040-1-AP targets TSNAX in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, IF, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TSNAX fusion protein Ag1507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name translin-associated factor X
Synonyms translin associated factor X, Translin associated protein X, TRAX, TSNAX
Calculated Molecular Weight 33 kDa
GenBank Accession NumberBC011797
Gene Symbol TSNAX
Gene ID (NCBI) 7257
RRIDAB_2303821
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99598
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for TSNAX antibody 11040-1-APDownload protocol
IF protocol for TSNAX antibody 11040-1-APDownload protocol
IP protocol for TSNAX antibody 11040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols