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TSPAN10 Polyclonal antibody

TSPAN10 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14430-1-AP

Print datasheet

Synonyms

Tspan-10, Tetraspanin-10, Oculospanin, OCSP

验证数据展示

  • WB analysis of hTERT-RPE1 using 14430-1-AP

    WB analysis of hTERT-RPE1 using 14430-1-AP

    WB result of TSPAN10 antibody (14430-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN10 transfected hTERT-RPE1 cells.

  • IF Staining of hTERT-RPE1 cells using 14430-1-AP

    IF Staining of hTERT-RPE1 cells using 14430-1-AP

    IF result (nuclear and the base of cilia stain) of anti-TSPAN10 (14430-1-AP, 1:50) with human hTERT-RPE1 (MeOH fixation) cells by Dr. Moshe Kim.

经过测试的应用

Positive WB detected inhTERT-RPE1 cells
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14430-1-AP targets TSPAN10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TSPAN10 fusion protein Ag5807 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 10
Synonyms Tspan-10, Tetraspanin-10, Oculospanin, OCSP
Calculated Molecular Weight 36.5 kDa
Observed Molecular Weight36-40 kDa
GenBank Accession NumberBC032802
Gene Symbol TSPAN10
Gene ID (NCBI) 83882
RRIDAB_2878057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1Z9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN10 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN10 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

实验方案

Product Specific Protocols
WB protocol for TSPAN10 antibody 14430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols