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验证数据展示

  • WB analysis of hTERT-RPE1 using 14430-1-AP

    WB analysis of hTERT-RPE1 using 14430-1-AP

    WB result of TSPAN10 antibody (14430-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TSPAN10 transfected hTERT-RPE1 cells.

  • IF Staining of hTERT-RPE1 cells using 14430-1-AP

    IF Staining of hTERT-RPE1 cells using 14430-1-AP

    IF result (nuclear and the base of cilia stain) of anti-TSPAN10 (14430-1-AP, 1:50) with human hTERT-RPE1 (MeOH fixation) cells by Dr. Moshe Kim.

  • Featured Product
  • KD/KO验证

TSPAN10 Polyclonal antibody

TSPAN10 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14430-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Tspan-10, Tetraspanin-10, Oculospanin, OCSP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2400
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14430-1-AP targets TSPAN10 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 TSPAN10 fusion protein Ag5807 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetraspanin 10
别名 Tspan-10, Tetraspanin-10, Oculospanin, OCSP
计算分子量 36.5 kDa
观测分子量36-40 kDa
GenBank蛋白编号BC032802
基因名称 TSPAN10
Gene ID (NCBI) 83882
RRIDAB_2878057
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H1Z9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN10 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN10 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN14, TSPAN15, TSPAN17, and TSPAN33. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

实验方案

Product Specific Protocols
WB protocol for TSPAN10 antibody 14430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols