验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12812-1-AP targets TSPAN12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | TSPAN12 fusion protein Ag3514 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | tetraspanin 12 |
| 别名 | NET 2, NET2, Tetraspan NET 2, tetraspanin 12, TM4SF12, Tspan 12, TSPAN12 |
| 计算分子量 | 305 aa, 35 kDa |
| 观测分子量 | 28 kDa |
| GenBank蛋白编号 | BC031265 |
| 基因名称 | TSPAN12 |
| Gene ID (NCBI) | 23554 |
| RRID | AB_2208667 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95859 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSPAN12 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN12 regulates retinal vascular development by promoting Norrin/beta-catenin signaling (PMID: 19837033 ). Mutations in TSPAN12 are a relatively frequent cause of familial exudative vitreoretinopathy (FEVR) (PMID: 20159111).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPAN12 antibody 12812-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
