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TSPAN12 Polyclonal antibody

TSPAN12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12812-1-AP

Print datasheet

Synonyms

NET 2, NET2, Tetraspan NET 2, tetraspanin 12, TM4SF12, Tspan 12, TSPAN12

验证数据展示

  • WB analysis of HepG2 using 12812-1-AP

    WB analysis of HepG2 using 12812-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 12812-1-AP (TSPAN12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12812-1-AP targets TSPAN12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TSPAN12 fusion protein Ag3514 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 12
Synonyms NET 2, NET2, Tetraspan NET 2, tetraspanin 12, TM4SF12, Tspan 12, TSPAN12
Calculated Molecular Weight 305 aa, 35 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC031265
Gene Symbol TSPAN12
Gene ID (NCBI) 23554
RRIDAB_2208667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95859
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN12 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN12 regulates retinal vascular development by promoting Norrin/beta-catenin signaling (PMID: 19837033 ). Mutations in TSPAN12 are a relatively frequent cause of familial exudative vitreoretinopathy (FEVR) (PMID: 20159111).

实验方案

Product Specific Protocols
WB protocol for TSPAN12 antibody 12812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols