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  • Featured Product
  • KD/KO Validated

TSPAN13 Polyclonal antibody

TSPAN13 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13570-1-AP

Print datasheet

Synonyms

Tspan-13, Transmembrane 4 superfamily member 13, TM4SF13, Tetraspanin-13, Tetraspan NET-6

验证数据展示

  • WB analysis of mouse lung using 13570-1-AP

    WB analysis of mouse lung using 13570-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13570-1-AP (TSPAN13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse lung using 13570-1-AP

    IP experiment of mouse lung using 13570-1-AP

    IP result of anti-TSPAN13 (IP:13570-1-AP, 3ug; Detection:13570-1-AP 1:300) with mouse lung tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13570-1-AP targets TSPAN13 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TSPAN13 fusion protein Ag4464 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 13
Synonyms Tspan-13, Transmembrane 4 superfamily member 13, TM4SF13, Tetraspanin-13, Tetraspan NET-6
Calculated Molecular Weight 204 aa, 22 kDa
Observed Molecular Weight 28-35 kDa
GenBank Accession NumberBC033863
Gene Symbol TSPAN13
Gene ID (NCBI) 27075
RRIDAB_10597858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95857
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN13 (also known as NET6 or TM4SF13) is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Many of these members have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. Overexpression of TSPAN13 has been reported in prostate cancer, suggesting that TSPAN13 may have an important role in the progression of prostate cancer (PMID: 19148481). TSPAN13 has a calcular molecular weight of 22 kDa. This polyclonal antibody is raised against 100-204aa of human TSPAN13. The experimentally determined molecular mass of 28-35 kDa is larger than the calculated molecular mass, which could be a result of post-translational modifications (PMID: 23648579).

实验方案

Product Specific Protocols
WB protocol for TSPAN13 antibody 13570-1-APDownload protocol
IP protocol for TSPAN13 antibody 13570-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Bosn J Basic Med Sci

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC).

Authors - Peng Li
  • KD Validated
View Article