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TSPAN3 Polyclonal antibody

TSPAN3 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13036-1-AP

Print datasheet

Synonyms

Tspan-3, Transmembrane 4 superfamily member 8, TM4SF8, Tetraspanin-3, Tetraspanin TM4-A

验证数据展示

  • IHC staining of mouse brain using 13036-1-AP

    IHC staining of mouse brain using 13036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13036-1-AP (TSPAN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 13036-1-AP

    IHC staining of mouse brain using 13036-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13036-1-AP (TSPAN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 13036-1-AP

    IF Staining of HeLa using 13036-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TSPAN3 antibody (13036-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13036-1-AP targets TSPAN3 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TSPAN3 fusion protein Ag3689 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 3
Synonyms Tspan-3, Transmembrane 4 superfamily member 8, TM4SF8, Tetraspanin-3, Tetraspanin TM4-A
Calculated Molecular Weight 253 aa, 28 kDa
GenBank Accession NumberBC011206
Gene Symbol TSPAN3
Gene ID (NCBI) 10099
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60637
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tetraspanin-3 (TSPAN3, also known as TM4SF8) is a member of the tetraspanin (TM4SF) family. Tetraspanins, so named because they contain four transmembrane domains, are frequently expressed at the cell surface in association with each other and with other molecules, and they function to regulate cell adhesion, migration, proliferation, and differentiation. TSPAN3 regulates the proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair. TSPAN3 is a glycosylated protein and it is differentially glycosylated in different tissues (PMID: 27818272).

实验方案

Product Specific Protocols
IHC protocol for TSPAN3 antibody 13036-1-APDownload protocol
IF protocol for TSPAN3 antibody 13036-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols