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验证数据展示

  • WB analysis of human brain using 14329-1-AP

    WB analysis of human brain using 14329-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14329-1-AP (TSPAN33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 14329-1-AP

    IHC staining of human hepatocirrhosis using 14329-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 14329-1-AP

    IHC staining of human hepatocirrhosis using 14329-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 40x lens).

TSPAN33 Polyclonal antibody

TSPAN33 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14329-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hPen, PEN, Penumbra, Proerythroblast new membrane, tetraspanin 33, Tspan 33, TSPAN33

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue, human plasma tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14329-1-AP targets TSPAN33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 TSPAN33 fusion protein Ag5597 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetraspanin 33
别名 hPen, PEN, Penumbra, Proerythroblast new membrane, tetraspanin 33, Tspan 33, TSPAN33
计算分子量 32 kDa
观测分子量 64-72 kDa
GenBank蛋白编号BC044244
基因名称 TSPAN33
Gene ID (NCBI) 340348
RRIDAB_10644331
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86UF1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN33 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN33 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN15, and TSPAN17. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

实验方案

Product Specific Protocols
WB protocol for TSPAN33 antibody 14329-1-APDownload protocol
IHC protocol for TSPAN33 antibody 14329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Immunol

The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage Activation through Modulation of NOTCH Signaling.

Authors - Almudena Ruiz-García
View Article