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TSPAN33 Polyclonal antibody

TSPAN33 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14329-1-AP

Print datasheet

Synonyms

hPen, PEN, Penumbra, Proerythroblast new membrane, tetraspanin 33, Tspan 33, TSPAN33

验证数据展示

  • WB analysis of human brain using 14329-1-AP

    WB analysis of human brain using 14329-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14329-1-AP (TSPAN33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 14329-1-AP

    IHC staining of human hepatocirrhosis using 14329-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human hepatocirrhosis using 14329-1-AP

    IHC staining of human hepatocirrhosis using 14329-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 14329-1-AP (TSPAN33 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inhuman brain tissue, human plasma tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14329-1-AP targets TSPAN33 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TSPAN33 fusion protein Ag5597 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetraspanin 33
Synonyms hPen, PEN, Penumbra, Proerythroblast new membrane, tetraspanin 33, Tspan 33, TSPAN33
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 64-72 kDa
GenBank Accession NumberBC044244
Gene Symbol TSPAN33
Gene ID (NCBI) 340348
RRIDAB_10644331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UF1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPAN33 is a membrane protein of the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. TSPAN33 belongs to the TSPANC8 subfamily, which also include TSPAN5, TSPAN10, TSPAN14, TSPAN15, and TSPAN17. It has been reported that TSPANC8 tetraspanins interact with ADAM10 and have a conserved function in the regulation of ADAM10 trafficking and activity, thereby positively regulating Notch receptor activation (PMID: 23091066; 23035126).

实验方案

Product Specific Protocols
WB protocol for TSPAN33 antibody 14329-1-APDownload protocol
IHC protocol for TSPAN33 antibody 14329-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Immunol

The Tetraspanin TSPAN33 Controls TLR-Triggered Macrophage Activation through Modulation of NOTCH Signaling.

Authors - Almudena Ruiz-García
View Article