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验证数据展示

  • IHC staining of mouse heart using 15444-1-AP

    IHC staining of mouse heart using 15444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15444-1-AP (TSPO/PBR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 15444-1-AP

    IHC staining of mouse heart using 15444-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15444-1-AP (TSPO antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HEK-293 using 15444-1-AP

    WB analysis of HEK-293 using 15444-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15444-1-AP (TSPO/PBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

TSPO/PBR Polyclonal antibody

TSPO/PBR Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15444-1-AP

Print datasheet

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

BZRP, DBI, IBP, MBR, mDRC, PBR, pk18, PKBS, PTBR, Translocator protein, translocator protein (18kDa), TSPO

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15444-1-AP targets TSPO/PBR in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen TSPO/PBR fusion protein Ag7475 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name translocator protein (18kDa)
Synonyms BZRP, DBI, IBP, MBR, mDRC, PBR, pk18, PKBS, PTBR, Translocator protein, translocator protein (18kDa), TSPO
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC001110
Gene Symbol TSPO
Gene ID (NCBI) 706
RRIDAB_3085462
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30536
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPO, also named Peripheral-type benzodiazepine receptor (PBR), encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).

实验方案

Product Specific Protocols
WB protocol for TSPO/PBR antibody 15444-1-APDownload protocol
IHC protocol for TSPO/PBR antibody 15444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Neurochem Res

The lncRNA NEAT1 Mediates Neuronal Cell Autophagy and Related Protein Expression After Cerebral Ischemia‒Reperfusion Injury

Authors - Wenbo Xia
View Article