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TSPO Monoclonal antibody, PBS Only

TSPO Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

rat, mouse, human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1D1F12

Cat no : 68137-1-PBS

Print datasheet

Synonyms

Peripheral-type benzodiazepine receptor, MBR, BZRP, 1D1F12

验证数据展示

  • WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    WB analysis using 68137-1-Ig (same clone as 68137-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • WB analysis of Jurkat using 68137-1-Ig (same clone as 68137-1-PBS)

    WB analysis of Jurkat using 68137-1-Ig (same clone as 68137-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68137-1-Ig (TSPO/PBR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 68137-1-Ig (same clone as 68137-1-PBS)

    IF Staining of NIH/3T3 using 68137-1-Ig (same clone as 68137-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using TSPO/PBR antibody (68137-1-Ig, Clone: 1D1F12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68137-1-PBS in a different storage buffer formulation.

产品信息

68137-1-PBS targets TSPO in WB, IF/ICC, ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen TSPO fusion protein Ag7475 种属同源性预测
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Full Name translocator protein (18kDa)
Synonyms Peripheral-type benzodiazepine receptor, MBR, BZRP, 1D1F12
Calculated Molecular Weight 18 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC001110
Gene Symbol TSPO
Gene ID (NCBI) 706
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30536
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

TSPO encodes the translocator protein, belonging to tryptophan-rich sensory protein family. The protein is mainly localized in the outer mitochondrial membrane and expressed in steroid-synthesizing tissues (PMID:32881658, PMID:1326278). TSPO is involved in the translocation of cholesterol from the outer to inner mitochondrial membrane (PMID:21119734). In response to injury, TSPO has a upregulated expression in the peripheral nervous system (PMID:32423330, PMID:23251719). TSPO is also as a biomarker in a non-invasive procedure including detecting the inflammation in the heart and atherosclerotic plaques (PMID:24402571).