• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

TSPY3 Polyclonal antibody

TSPY3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22257-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 78, CT78, TSPY, TSPY1, TSPY3

验证数据展示

  • WB analysis of human testis using 22257-1-AP

    WB analysis of human testis using 22257-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 22257-1-AP (TSPY3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 22257-1-AP

    IHC staining of human testis using 22257-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 22257-1-AP (TSPY3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of rat testis using 22257-1-AP

    IHC staining of rat testis using 22257-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 22257-1-AP (TSPY3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman testis tissue, LNCaP cells
Positive IHC detected inhuman testis tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22257-1-AP targets TSPY3 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, rat
Immunogen TSPY3 fusion protein Ag17762 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name testis specific protein, Y-linked 3
Synonyms Cancer/testis antigen 78, CT78, TSPY, TSPY1, TSPY3
Calculated Molecular Weight 308 aa, 35 kDa
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC075016
Gene Symbol TSPY3
Gene ID (NCBI) 728137
RRIDAB_11182707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP0CV98
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSPY3 antibody 22257-1-APDownload protocol
IHC protocol for TSPY3 antibody 22257-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols