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  • Featured Product
  • KD/KO Validated

CDA1 Polyclonal antibody

CDA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 12087-2-AP

Print datasheet

Synonyms

TSPX, TSPYL2, DENTT, Cutaneous T-cell lymphoma-associated antigen se20-4, CTCL-associated antigen se20-4

验证数据展示

  • WB analysis of HeLa using 12087-2-AP

    WB analysis of HeLa using 12087-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12087-2-AP (CDA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12087-2-AP

    IHC staining of human lung cancer using 12087-2-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12087-2-AP (CDA1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 12087-2-AP

    IF Staining of HeLa using 12087-2-AP

    Immunofluorescent analysis of Hela cells, using TSPYL2 antibody 12087-2-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of HEK-293 using 12087-2-AP

    IP experiment of HEK-293 using 12087-2-AP

    IP result of anti-CDA1 (IP:12087-2-AP, 4ug; Detection:12087-2-AP 1:1000) with HEK-293 cells lysate 1000ug.

经过测试的应用

Positive WB detected inHeLa cells, DU 145 cells, HEK-293 cells, MCF-7 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12087-2-AP targets CDA1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, CoIP, IF
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen CDA1 fusion protein Ag2722 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TSPY-like 2
Synonyms TSPX, TSPYL2, DENTT, Cutaneous T-cell lymphoma-associated antigen se20-4, CTCL-associated antigen se20-4
Calculated Molecular Weight 693 aa, 79 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC024270
Gene Symbol CDA1
Gene ID (NCBI) 64061
RRIDAB_2256811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2G4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TSPYL2 (also known as CINAP, CDA1, TSPX or DENTT) is a new member of the nucleosome assembly protein superfamily. TSPYL2 binds histones and facilitates nucleosome assembly. TSPYL2 is expressed in various tissues, highly in the pituitary gland and moderately in the adrenals, brain, testis, and ovary. Immunohistochemical staining analysis for TSPYL2 showed differential cytoplasmic and nuclear staining patterns in several cell types. Downregulated expression of TSPYL2 has been observed in several tumors, which suggests its role as a tumor suppressor. Although it is predicted that TSPYL2 has a molecular mass of 79.43 kDa, it is found that mammalian TSPYL2 appears at a size of 120 kDa by western blot analysis. The abundant acidic amino acid regions in TSPYL2 may cause its aberrant migration. In addition, the TSPYL2 protein is unstable and sensitive to proteasomal degradation.

实验方案

Product Specific Protocols
WB protocol for CDA1 antibody 12087-2-APDownload protocol
IHC protocol for CDA1 antibody 12087-2-APDownload protocol
IF protocol for CDA1 antibody 12087-2-APDownload protocol
IP protocol for CDA1 antibody 12087-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Glioma stem cell proliferation and tumor growth are promoted by nitric oxide synthase-2.

Authors - Eyler Christine E CE
View Article
humanWB

Cell Death Differ

TSPYL2 is an essential component of the REST/NRSF transcriptional complex for TGFβ signaling activation.

Authors - M T Epping
  • KD Validated
View Article
humanWB

PLoS One

The X-linked tumor suppressor TSPX interacts and promotes degradation of the hepatitis B viral protein HBx via the proteasome pathway.

Authors - Kido Tatsuo T
View Article
humanIF

Stem Cells Int

Expression of Genes Related to Germ Cell Lineage and Pluripotency in Single Cells and Colonies of Human Adult Germ Stem Cells.

Authors - Sabine Conrad
View Article
humanWB

Oncotarget

The X-linked tumor suppressor TSPX downregulates cancer-drivers/oncogenes in prostate cancer in a C-terminal acidic domain dependent manner.

Authors - Tatsuo Kido
View Article
humanWB,IF

J Ginseng Res

Ginsenoside Rg3 increases gemcitabine sensitivity of pancreatic adenocarcinoma via reducing ZFP91 mediated TSPYL2 destabilization

Authors - Haixia Pan
View Article