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TSR1 Polyclonal antibody

TSR1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29957-1-AP

Print datasheet

Synonyms

Pre-rRNA-processing protein TSR1 homolog, KIAA1401, FLJ10534

验证数据展示

  • WB analysis using 29957-1-AP

    WB analysis using 29957-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29957-1-AP (TSR1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-251 using 29957-1-AP

    IF Staining of U-251 using 29957-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using TSR1 antibody (29957-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, L02 cells, U-251 cells
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29957-1-AP targets TSR1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen TSR1 fusion protein Ag31575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name TSR1, 20S rRNA accumulation, homolog (S. cerevisiae)
Synonyms Pre-rRNA-processing protein TSR1 homolog, KIAA1401, FLJ10534
Calculated Molecular Weight92KD
Observed Molecular Weight 100 kDa
GenBank Accession NumberNM_018128
Gene Symbol TSR1
Gene ID (NCBI) 55720
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2NL82
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSR1 antibody 29957-1-APDownload protocol
IF protocol for TSR1 antibody 29957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols