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TSSK3 Polyclonal antibody

TSSK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12940-1-AP

Print datasheet

Synonyms

SPOGA3, STK22C, STK22D, testis serine kinase 3, Testis specific kinase 3, TSK 3, TSSK 3, TSSK3

验证数据展示

  • WB analysis of mouse testis using 12940-1-AP

    WB analysis of mouse testis using 12940-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12940-1-AP (TSSK3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12940-1-AP

    IP experiment of mouse testis using 12940-1-AP

    IP result of anti-TSSK3 (IP:12940-1-AP, 4ug; Detection:12940-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • IHC staining of human liver cancer using 12940-1-AP

    IHC staining of human liver cancer using 12940-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12940-1-AP (TSSK3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 12940-1-AP

    IHC staining of human liver cancer using 12940-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12940-1-AP (TSSK3 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12940-1-AP targets TSSK3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen TSSK3 fusion protein Ag4009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name testis-specific serine kinase 3
Synonyms SPOGA3, STK22C, STK22D, testis serine kinase 3, Testis specific kinase 3, TSK 3, TSSK 3, TSSK3
Calculated Molecular Weight 268 aa, 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC035354
Gene Symbol TSSK3
Gene ID (NCBI) 81629
RRIDAB_2877894
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96PN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSSK3 antibody 12940-1-APDownload protocol
IHC protocol for TSSK3 antibody 12940-1-APDownload protocol
IP protocol for TSSK3 antibody 12940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Andrology

Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility

Authors - Kaori Nozawa
  • KO Validated
View Article
mouseWB

Andrology

CUB domains are not required for OVCH2 function in sperm maturation in the mouse epididymis

Authors - Katarzyna Kent
View Article