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验证数据展示

  • WB analysis of mouse testis using 12940-1-AP

    WB analysis of mouse testis using 12940-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12940-1-AP (TSSK3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 12940-1-AP

    IP experiment of mouse testis using 12940-1-AP

    IP result of anti-TSSK3 (IP:12940-1-AP, 4ug; Detection:12940-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • IHC staining of human liver cancer using 12940-1-AP

    IHC staining of human liver cancer using 12940-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12940-1-AP (TSSK3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver cancer using 12940-1-AP

    IHC staining of human liver cancer using 12940-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12940-1-AP (TSSK3 antibody) at dilution of 1:100 (under 40x lens).

TSSK3 Polyclonal antibody

TSSK3 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12940-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

SPOGA3, STK22C, STK22D, testis serine kinase 3, Testis specific kinase 3, TSK 3, TSSK 3, TSSK3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12940-1-AP targets TSSK3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 TSSK3 fusion protein Ag4009 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 testis-specific serine kinase 3
别名 SPOGA3, STK22C, STK22D, testis serine kinase 3, Testis specific kinase 3, TSK 3, TSSK 3, TSSK3
计算分子量 268 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC035354
基因名称 TSSK3
Gene ID (NCBI) 81629
RRIDAB_2877894
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PN8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSSK3 antibody 12940-1-APDownload protocol
IHC protocol for TSSK3 antibody 12940-1-APDownload protocol
IP protocol for TSSK3 antibody 12940-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Andrology

Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility

Authors - Kaori Nozawa
  • KO Validated
View Article
mouseWB

Andrology

CUB domains are not required for OVCH2 function in sperm maturation in the mouse epididymis

Authors - Katarzyna Kent
View Article