验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HT-29 cells, COLO 320 cells, mouse testis tissue, mouse colon tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83401-4-RR targets TSSK4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | TSSK4 fusion protein Ag16205 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | testis-specific serine kinase 4 |
| 别名 | testis serine kinase 4, STK22E, Serine/threonine-protein kinase 22E, EC:2.7.11.1, C14orf20 |
| 计算分子量 | 338 aa, 37 kDa |
| 观测分子量 | 38-45 kDa |
| GenBank蛋白编号 | BC111088 |
| 基因名称 | TSSK4 |
| Gene ID (NCBI) | 283629 |
| RRID | AB_3671052 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q6SA08 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TSSK4 (Testis-specific serine/threonine-protein kinase 4), testis-specific serine/threonine protein kinase 4, is a member of the testis-specific serine/threonine protein kinase family. It has autophosphorylation activity and self-binding properties; it can interact with and phosphorylate other proteins, e.g., it can bind to and phosphorylate Odf2. It's reported that TSSK4 plays an important role in spermatogenesis, TSSK4 knockout male mice develop reduced sperm motility and reduced fertility. In addition, TSSK4 regulates lung fibrosis by promoting the loss of alveolar epithelial type II cells through the HSP90-AKT signaling pathway.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TSSK4 antibody 83401-4-RR | Download protocol |
| IHC protocol for TSSK4 antibody 83401-4-RR | Download protocol |
| IF protocol for TSSK4 antibody 83401-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |