TST Monoclonal antibody
TST Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2A6C5
验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, human brain tissue, mouse brain tissue, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy TST Ready-To-Use IHC Kit. TST primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IHC | See 1 publications below |
产品信息
66018-1-Ig targets TST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | TST fusion protein Ag9235 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | thiosulfate sulfurtransferase (rhodanese) |
| Synonyms | Rhodanese, RDS, EC:2.8.1.1, 2A6C5 |
| Calculated Molecular Weight | 297 aa, 33 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC010148 |
| Gene Symbol | TST |
| Gene ID (NCBI) | 7263 |
| RRID | AB_11045660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16762 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Thiosulfate sulfurtransferase (TST), also known as rhodanese, is a mitochondrial enzyme involved in cyanide detoxification and the formation of iron-sulfur proteins and the modification of sulfur-containing enzymes.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TST antibody 66018-1-Ig | Download protocol |
| IHC protocol for TST antibody 66018-1-Ig | Download protocol |
| IF protocol for TST antibody 66018-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Polymorphic variants of human rhodanese exhibit differences in thermal stability and sulfurtransfer kinetics. | ||
Biochem Pharmacol Drug resistance induces the upregulation of H2S-producing enzymes in HCT116 colon cancer cells. | ||
J Biol Chem Hydrogen sulfide perturbs mitochondrial bioenergetics and triggers metabolic reprogramming in colon cells. | ||
Biomolecules Sulfurtransferases and Cystathionine Beta-Synthase Expression in Different Human Leukemia Cell Lines. | ||
Mol Biol Rep Curcumin reduces myocardial ischemia-reperfusion injury, by increasing endogenous H2S levels and further modulating m6A | ||
Burns Cystathionine-gamma-lyase deficient mice are protected against the development of multiorgan failure and exhibit reduced inflammatory response during burn. |
