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验证数据展示

  • WB analysis of human colon using 15335-1-AP

    WB analysis of human colon using 15335-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 15335-1-AP (TSTA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 15335-1-AP

    IHC staining of human kidney using 15335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15335-1-AP (TSTA3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human kidney using 15335-1-AP

    IHC staining of human kidney using 15335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15335-1-AP (TSTA3 Antibody) at dilution of 1:200 (under 40x lens).

TSTA3 Polyclonal antibody

TSTA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15335-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

FX, GDP L fucose synthase, P35B, Protein FX, SDR4E1, TSTA3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman colon tissue, HEK-293 cells, HeLa cells, human brain tissue, human kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15335-1-AP targets TSTA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 TSTA3 fusion protein Ag7450 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tissue specific transplantation antigen P35B
别名 FX, GDP L fucose synthase, P35B, Protein FX, SDR4E1, TSTA3
计算分子量 36 kDa
观测分子量 40 kDa, 68 kDa
GenBank蛋白编号BC001941
基因名称 TSTA3
Gene ID (NCBI) 7264
RRIDAB_2211226
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13630
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TSTA3 antibody 15335-1-APDownload protocol
IHC protocol for TSTA3 antibody 15335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols