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TTC28 Polyclonal antibody

TTC28 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25701-1-AP

Print datasheet

Synonyms

KIAA1043, TPR repeat protein 28, TTC28

验证数据展示

  • WB analysis using 25701-1-AP

    WB analysis using 25701-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25701-1-AP (TTC28 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 25701-1-AP

    WB analysis of HeLa using 25701-1-AP

    WB result of TTC28 antibody (25701-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC28 transfected HeLa cells.

  • IP experiment of HEK-293 using 25701-1-AP

    IP experiment of HEK-293 using 25701-1-AP

    IP result of anti-TTC28 (IP:25701-1-AP, 4ug; Detection:25701-1-AP 1:2500) with HEK-293 cells lysate 1480 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25701-1-AP targets TTC28 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human
Immunogen TTC28 fusion protein Ag22465 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 28
Synonyms KIAA1043, TPR repeat protein 28, TTC28
Calculated Molecular Weight 2481 aa, 271 kDa
Observed Molecular Weight240-271 kDa
GenBank Accession NumberBC016465
Gene Symbol TTC28
Gene ID (NCBI) 23331
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC28 is a large protein required during the cell cycle for condensation of spindle midzone microtubules, formation of the midbody, and completion of cytokinesis. RT-PCR ELISA detected highest TTC28 expression in adult ovary and lung, followed by kidney and liver. Lower expression was detected in all other adult and fetal tissues examined except testis, which showed no expression.

实验方案

Product Specific Protocols
WB protocol for TTC28 antibody 25701-1-APDownload protocol
IP protocol for TTC28 antibody 25701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols