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TTC32 Polyclonal antibody

TTC32 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25422-1-AP

Print datasheet

Synonyms

TPR repeat protein 32, TTC32

验证数据展示

  • IHC staining of mouse testis using 25422-1-AP

    IHC staining of mouse testis using 25422-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25422-1-AP (TTC32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 25422-1-AP

    WB analysis of mouse testis using 25422-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 25422-1-AP (TTC32 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 25422-1-AP

    IP experiment of mouse testis using 25422-1-AP

    IP result of anti-TTC32 (IP:25422-1-AP, 4ug; Detection:25422-1-AP 1:300) with mouse testis tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25422-1-AP targets TTC32 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen TTC32 fusion protein Ag22115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 32
Synonyms TPR repeat protein 32, TTC32
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 17-30 kDa
GenBank Accession NumberBC057850
Gene Symbol TTC32
Gene ID (NCBI) 130502
RRIDAB_2880073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5I0X7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC32 antibody 25422-1-APDownload protocol
IHC protocol for TTC32 antibody 25422-1-APDownload protocol
IP protocol for TTC32 antibody 25422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols