验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse heart tissue, K-562 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, human placenta tissue, PC-3 cells, SKOV-3 cells |
| Positive IP detected in | mouse heart tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25443-1-AP targets TTC35 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, ELISA Application Description |
| Cited Applications | WB, IP, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | TTC35 fusion protein Ag22196 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | tetratricopeptide repeat domain 35 |
| Synonyms | EMC2, KIAA0103, TPR repeat protein 35, TTC35 |
| Calculated Molecular Weight | 297 aa, 35 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC021667 |
| Gene Symbol | TTC35 |
| Gene ID (NCBI) | 9694 |
| RRID | AB_2750836 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15006 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPR repeat protein 35 (TTC35), also named as tetratricopeptide repeat domain 35, is a 297 amino acid protein, which contains three TPR repeats and belongs to the EMC2 family. TTC35 localizes in the nucleus and cytoplasm. TTC35 is a component of the ER membrane protein complex and may be a putative O-linked glycosyl transferase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TTC35 antibody 25443-1-AP | Download protocol |
| IP protocol for TTC35 antibody 25443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. | ||
J Cell Biol A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER | ||
Elife The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins. | ||
Elife Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
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