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TTC35 Polyclonal antibody

TTC35 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25443-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA and More (1)

EMC2, KIAA0103, TPR repeat protein 35, TTC35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, mouse heart tissue, K-562 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, human placenta tissue, PC-3 cells, SKOV-3 cells
Positive IP detected inmouse heart tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25443-1-AP targets TTC35 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IP, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TTC35 fusion protein Ag22196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tetratricopeptide repeat domain 35
别名 EMC2, KIAA0103, TPR repeat protein 35, TTC35
计算分子量 297 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC021667
基因名称 TTC35
Gene ID (NCBI) 9694
RRIDAB_2750836
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15006
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TPR repeat protein 35 (TTC35), also named as tetratricopeptide repeat domain 35, is a 297 amino acid protein, which contains three TPR repeats and belongs to the EMC2 family. TTC35 localizes in the nucleus and cytoplasm. TTC35 is a component of the ER membrane protein complex and may be a putative O-linked glycosyl transferase.

实验方案

Product Specific Protocols
WB protocol for TTC35 antibody 25443-1-APDownload protocol
IP protocol for TTC35 antibody 25443-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

EMC Is Required to Initiate Accurate Membrane Protein Topogenesis.

Authors - Patrick J Chitwood
humanWB

Mol Cell

WNK1 is an assembly factor for the human ER membrane protein complex.

Authors - Tino Pleiner
humanWB

EMBO Mol Med

SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome.

Authors - Alexandre Janer
humanWB

J Cell Biol

A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER

Authors - Tino Pleiner
humanWB

Elife

The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins.

Authors - Ashley M Ngo
humanWB

Elife

Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.

Authors - Lakshmi E Miller-Vedam
  • KO Validated
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