验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, mouse heart tissue, K-562 cells, MCF-7 cells, NIH/3T3 cells, mouse spleen tissue, human placenta tissue, PC-3 cells, SKOV-3 cells |
Positive IP detected in | mouse heart tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25443-1-AP targets TTC35 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IP, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | TTC35 fusion protein Ag22196 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | tetratricopeptide repeat domain 35 |
别名 | EMC2, KIAA0103, TPR repeat protein 35, TTC35 |
计算分子量 | 297 aa, 35 kDa |
观测分子量 | 35 kDa |
GenBank蛋白编号 | BC021667 |
基因名称 | TTC35 |
Gene ID (NCBI) | 9694 |
RRID | AB_2750836 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q15006 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TPR repeat protein 35 (TTC35), also named as tetratricopeptide repeat domain 35, is a 297 amino acid protein, which contains three TPR repeats and belongs to the EMC2 family. TTC35 localizes in the nucleus and cytoplasm. TTC35 is a component of the ER membrane protein complex and may be a putative O-linked glycosyl transferase.
实验方案
Product Specific Protocols | |
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WB protocol for TTC35 antibody 25443-1-AP | Download protocol |
IP protocol for TTC35 antibody 25443-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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EMBO Mol Med SLC25A46 is required for mitochondrial lipid homeostasis and cristae maintenance and is responsible for Leigh syndrome. | ||
J Cell Biol A selectivity filter in the ER membrane protein complex limits protein misinsertion at the ER | ||
Elife The ER membrane protein complex is required to ensure correct topology and stable expression of flavivirus polyproteins. | ||
Elife Structural and mechanistic basis of the EMC-dependent biogenesis of distinct transmembrane clients.
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