TTC37 Polyclonal antibody

TTC37 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24594-1-AP

Print datasheet

Synonyms

TPR repeat protein 37, Thespin, Superkiller complex protein 3, SKIC3, Ski3



经过测试的应用

Positive WB detected inHEK-293 cells, MCF-7 cells, HeLa cells, HepG2 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman kidney tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24594-1-AP targets TTC37 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen TTC37 fusion protein Ag18442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 37
Synonyms TPR repeat protein 37, Thespin, Superkiller complex protein 3, SKIC3, Ski3
Calculated Molecular Weight 1564 aa, 175 kDa
Observed Molecular Weight 140-150 kDa
GenBank Accession NumberBC056893
Gene Symbol TTC37
Gene ID (NCBI) 9652
RRIDAB_2879628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PGP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTC37 (tetratricopeptide repeat protein 37), also named as Thespin, encodes 1,564 amino acid protein that contains twenty tetratricopeptide (TPR) repeats. TPR repeat-containing motifs are found in a variety of proteins and may mediate protein-protein interactions and chaperone activity. Although not expressed in liver, TTC37 is a widely expressed protein with highest levels of expression in vascular tissues, lymph node, pituitary, lung and intestine. TTC37 is a component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1 complex (PAF1C). Defects in TTC37 are associated with Trico-Hepato-Enteric (THE) syndrome.

实验方案

Product Specific Protocols
WB protocol for TTC37 antibody 24594-1-APDownload protocol
IHC protocol for TTC37 antibody 24594-1-APDownload protocol
IF protocol for TTC37 antibody 24594-1-APDownload protocol
IP protocol for TTC37 antibody 24594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

J Clin Invest

Cytoplasmic RNA quality control failure engages mTORC1-mediated autoinflammatory disease.

Authors - Kun Yang
humanWB

PLoS Genet

A Comprehensive Genomic Analysis Reveals the Genetic Landscape of Mitochondrial Respiratory Chain Complex Deficiencies.

Authors - Masakazu Kohda
humanWB

Mol Cancer Ther

Targeting the synthetic lethal relationship between FOCAD and TUT7 represents a potential therapeutic opportunity for TUT4/7 small molecule inhibitors in cancer

Authors - Robinson Triboulet
humanWB

EMBO J

RNA helicase SKIV2L limits antiviral defense and autoinflammation elicited by the OAS-RNase L pathway

Authors - Kun Yang
  • KO Validated