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TTC4 Polyclonal antibody

TTC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11878-1-AP

Print datasheet

Synonyms

TPR repeat protein 4, TTC4

验证数据展示

  • WB analysis of HeLa using 11878-1-AP

    WB analysis of HeLa using 11878-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11878-1-AP (TTC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lymphoma using 11878-1-AP

    IHC staining of human lymphoma using 11878-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11878-1-AP (TTC4 antibody) at dilution of 1:50 (under 10x lens).

  • WB analysis of HEK-293 using 11878-1-AP

    WB analysis of HEK-293 using 11878-1-AP

    WB result of TTC4 antibody (11878-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTC4 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11878-1-AP targets TTC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen TTC4 fusion protein Ag2474 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 4
Synonyms TPR repeat protein 4, TTC4
Calculated Molecular Weight 387 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC001276
Gene Symbol TTC4
Gene ID (NCBI) 7268
RRIDAB_2211700
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95801
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTC4 antibody 11878-1-APDownload protocol
IHC protocol for TTC4 antibody 11878-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols