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验证数据展示

  • WB analysis of mouse testis using 12505-1-AP

    WB analysis of mouse testis using 12505-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12505-1-AP (BBS8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12505-1-AP

    IP experiment of HeLa using 12505-1-AP

    IP result of anti-BBS8 (IP:12505-1-AP, 3ug; Detection:12505-1-AP 1:400) with HeLa cells lysate 2000ug.

BBS8 Polyclonal antibody

BBS8 Polyclonal Antibody for WB, IP, ELISA
Cat No. 12505-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

BBS8, TPR repeat protein 8, TTC8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, human lung tissue, NIH/3T3 cells
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12505-1-AP targets BBS8 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen BBS8 fusion protein Ag3186 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tetratricopeptide repeat domain 8
Synonyms BBS8, TPR repeat protein 8, TTC8
Calculated Molecular Weight 531 aa, 60 kDa
Observed Molecular Weight 50-52 kDa
GenBank Accession NumberBC026351
Gene Symbol BBS8
Gene ID (NCBI) 123016
RRIDAB_2211953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAM2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.

实验方案

Product Specific Protocols
WB protocol for BBS8 antibody 12505-1-APDownload protocol
IP protocol for BBS8 antibody 12505-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols