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验证数据展示

  • WB analysis of HeLa using 60235-1-Ig

    WB analysis of HeLa using 60235-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60235-1-Ig (transcription termination factor-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

transcription termination factor-Specific Monoclonal antibody

transcription termination factor-Specific Monoclonal Antibody for WB, ELISA
Cat No. 60235-1-Ig

产品说明书

CloneNo. 7B3H2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, ELISA

别名

TTF 1, TTF I, TTF1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHela cell

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60235-1-Ig targets transcription termination factor-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 transcription termination factor, RNA polymerase I
别名 TTF 1, TTF I, TTF1
计算分子量 103 kDa
观测分子量 106 kDa
GenBank蛋白编号NM_007344
基因名称 TTF1
Gene ID (NCBI) 7270
RRIDAB_2881359
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15361
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTF1 is a multifunctional nucleolar protein that terminates ribosomal gene transcription, mediates replication fork arrest and regulates RNA polymerase I transcription on chromatin. It plays a dual role in rDNA regulation, being involved in both activation and silencing of rDNA transcription. Interaction with BAZ2A/TIP5, TTF1 recovers DNA-binding activity. The antibody is specific to TTF1.

实验方案

Product Specific Protocols
WB protocol for transcription termination factor-Specific antibody 60235-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols