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TTF2 Polyclonal antibody

TTF2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13722-1-AP

Print datasheet

Synonyms

HuF2, F2, EC:3.6.4.-

验证数据展示

  • WB analysis of A2780 using 13722-1-AP

    WB analysis of A2780 using 13722-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 13722-1-AP

    IHC staining of human ovary tumor using 13722-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13722-1-AP (TTF2 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with TTF2 antibody included
  • IP experiment of A2780 using 13722-1-AP

    IP experiment of A2780 using 13722-1-AP

    IP result of anti-TTF2 (IP:13722-1-AP, 4ug; Detection:13722-1-AP 1:500) with A2780 cells lysate 840ug.

  • WB analysis using 13722-1-AP

    WB analysis using 13722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13722-1-AP (TTF2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 13722-1-AP

    IF Staining of HeLa using 13722-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (black).

  • IF Staining of HeLa using 13722-1-AP

    IF Staining of HeLa using 13722-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTF2 antibody (13722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inA2780 cells, HeLa cells, Jurkat cells
Positive IP detected inA2780 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TTF2 Ready-To-Use IHC Kit. TTF2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13722-1-AP targets TTF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Immunogen TTF2 fusion protein Ag4671 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name transcription termination factor, RNA polymerase II
Synonyms HuF2, F2, EC:3.6.4.-
Calculated Molecular Weight 1162 aa, 129 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC030058
Gene Symbol TTF2
Gene ID (NCBI) 8458
RRIDAB_2262865
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UNY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Transcription elongation is an key target for control of eukaryotic gene expression. After initiating from a promoter, the RNA polymerase II elongation complex is subjected to a block to elongation by the action of components of N-TEF (negative transcription elongation factor) [PMID: 9748214]. Transcription termination factor 2 (TTF2) is a DsDNA-dependent ATPase that acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. It also contribute to mitotic transcription repression, and involved in pre-mRNA splicing[PMID:15125840, 12927788].

实验方案

Product Specific Protocols
WB protocol for TTF2 antibody 13722-1-APDownload protocol
IHC protocol for TTF2 antibody 13722-1-APDownload protocol
IF protocol for TTF2 antibody 13722-1-APDownload protocol
IP protocol for TTF2 antibody 13722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

mRNA decapping factors and the exonuclease Xrn2 function in widespread premature termination of RNA polymerase II transcription.

Authors - Brannan Kris K
View Article
ratWB

Sci Total Environ

PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.

Authors - Mei Ha
View Article
humanIHC

Nat Commun

Single-cell analysis of gastric signet ring cell carcinoma reveals cytological and immune microenvironment features

Authors - Weizhu Zhao
View Article