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Species Reactivity
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Species Reactivity
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0
Conjugate
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验证数据展示

  • WB analysis of HEK-293 using 10381-1-AP (same clone as 10381-1-PBS)

    WB analysis of HEK-293 using 10381-1-AP (same clone as 10381-1-PBS)

    WB result of TTK antibody (10381-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-TTK transfected HEK-293 cells. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • WB analysis using 10381-1-AP (same clone as 10381-1-PBS)

    WB analysis using 10381-1-AP (same clone as 10381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10381-1-AP (TTK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 10381-1-AP (same clone as 10381-1-PBS)

    IHC staining of human breast cancer using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10381-1-AP (TTK Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TTK antibody included
  • IP experiment of DU 145 using 10381-1-AP (same clone as 10381-1-PBS)

    IP experiment of DU 145 using 10381-1-AP (same clone as 10381-1-PBS)

    IP result of anti-TTK (IP:10381-1-AP, 4ug; Detection:10381-1-AP 1:600) with DU 145 cells lysate 1280 ug. This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 10381-1-AP (same clone as 10381-1-PBS)

    IHC staining of mouse colon using 10381-1-AP (same clone as 10381-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 10381-1-AP (TTK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TTK antibody included
  • IF Staining of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

    IF Staining of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TTK antibody (10381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

    FC experiment of HeLa using 10381-1-AP (same clone as 10381-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human TTK (10381-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10381-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

TTK Polyclonal antibody, PBS Only

TTK Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 10381-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

MPS1L1, MPS1, Mps 1, hMPS1, ESK

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy TTK Ready-To-Use IHC Kit. TTK primary antibody included.

产品信息

10381-1-PBS targets TTK in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 TTK fusion protein Ag0405 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 TTK protein kinase
别名 MPS1L1, MPS1, Mps 1, hMPS1, ESK
计算分子量 97 kDa
观测分子量 96-97 kDa
GenBank蛋白编号BC000633
基因名称 TTK
Gene ID (NCBI) 7272
RRIDAB_2211979
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33981
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mps1 family of kinases colocalizes with mitotic checkpoint proteins in kinetochores and/or spindle pole bodies/centrosomes. TTK has been shown to be a cell cycle-regulated kinase displaying maximum activity during M phase(PMID:15618221). Its kinase activity is required for proper chromosome segregation during mitosis through its involvements in microtubule-chromosome attachment error correction and the mitotic checkpoint(PMID:20937696). TTK mRNA is present at relatively high levels in testis and thymus, tissues which contain a large number of proliferating cells, but is not detected in most other benign tissues (PMID:1639825). It has 2 isoforms produced by alternative splicing (PMID:25137017).