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验证数据展示

  • WB analysis of rat liver using 67431-1-Ig

    WB analysis of rat liver using 67431-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 67431-1-Ig (TTPA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of human liver cancer using 67431-1-Ig

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of human liver cancer using 67431-1-Ig

    IF Staining of human liver cancer using 67431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using TTPA antibody (67431-1-Ig, Clone: 3E7D10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human liver cancer using 67431-1-Ig

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTPA/TPP1 antibody included
  • IHC staining of human liver cancer using 67431-1-Ig

    IHC staining of human liver cancer using 67431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67431-1-Ig (TTPA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TTPA/TPP1 antibody included

TTPA Monoclonal antibody

TTPA Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67431-1-Ig

产品说明书

CloneNo. 3E7D10

宿主/亚型

Mouse / IgG2b

种属反应性

Human, Mouse, Rat, Pig

应用

WB, IHC, IF-P, ELISA

别名

Alpha TTP, alphaTTP, ATTP, AVED, TPP1, TTP1, TTPA

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue, pig liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy TTPA/TPP1 Ready-To-Use IHC Kit. TTPA/TPP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67431-1-Ig targets TTPA in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, Mouse, Rat, Pig
免疫原 TTPA fusion protein Ag13587 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 tocopherol (alpha) transfer protein
别名 Alpha TTP, alphaTTP, ATTP, AVED, TPP1, TTP1, TTPA
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC058000
基因名称 TTPA
Gene ID (NCBI) 7274
RRIDAB_2882669
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP49638
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TTPA antibody 67431-1-IgDownload protocol
IHC protocol for TTPA antibody 67431-1-IgDownload protocol
IF protocol for TTPA antibody 67431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols