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TTYH1 Polyclonal antibody

TTYH1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26973-1-AP

Print datasheet

Synonyms

hTTY1, Protein tweety homolog 1, TTYH1, tweety homolog 1 (Drosophila)

验证数据展示

  • WB analysis using 26973-1-AP

    WB analysis using 26973-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26973-1-AP (TTYH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 26973-1-AP

    IHC staining of human gliomas using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 26973-1-AP

    IHC staining of human gliomas using 26973-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 26973-1-AP (TTYH1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman gliomas
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26973-1-AP targets TTYH1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen TTYH1 fusion protein Ag25290 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tweety homolog 1 (Drosophila)
Synonyms hTTY1, Protein tweety homolog 1, TTYH1, tweety homolog 1 (Drosophila)
Calculated Molecular Weight 49-50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC011347
Gene Symbol TTYH1
Gene ID (NCBI) 57348
RRIDAB_2880707
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TTYH1, protein tweety homolog 1, is a glycosylated protein composed of five predicted transmembrane segments, which belongs to the tweety family. The expression of TTYH1 is restricted mainly to neural tissue and is up-regulated in astrocytoma, glioma and several other cancers. TTYH1 has some isoforms with MW of 49-50 kDa, 35 kDa and 26 kDa.

实验方案

Product Specific Protocols
WB protocol for TTYH1 antibody 26973-1-APDownload protocol
IHC protocol for TTYH1 antibody 26973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Elevated expression of Tweety homologue 3 predicts poor clinical outcomes in ovarian cancer.

Authors - Junhan Zhou
View Article