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验证数据展示

  • WB analysis of mouse brain using 17928-1-AP

    WB analysis of mouse brain using 17928-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17928-1-AP (TUB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 17928-1-AP

    IHC staining of human brain using 17928-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17928-1-AP (TUB antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 17928-1-AP

    IHC staining of human brain using 17928-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17928-1-AP (TUB antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of mouse brain using 17928-1-AP

    IP experiment of mouse brain using 17928-1-AP

    IP result of anti-TUB (IP:17928-1-AP, 4ug; Detection:17928-1-AP 1:1000) with mouse brain tissue lysate 3680ug.

  • Featured Product
  • KD/KO验证

TUB Polyclonal antibody

TUB Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17928-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (2)

别名

rd5, TUB, tubby homolog (mouse), Tubby protein homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17928-1-AP targets TUB in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IF, ChIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 TUB fusion protein Ag12354 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubby homolog (mouse)
别名 rd5, TUB, tubby homolog (mouse), Tubby protein homolog
计算分子量 561 aa, 62 kDa
观测分子量 56 kDa, 62-67 kDa
GenBank蛋白编号BC075032
基因名称 TUB
Gene ID (NCBI) 7275
RRIDAB_2212236
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50607
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TUB antibody 17928-1-APDownload protocol
IHC protocol for TUB antibody 17928-1-APDownload protocol
IP protocol for TUB antibody 17928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cells

Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Authors - Julie J Hong
View Article
humanChIP,WB

Cell Rep

NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.

Authors - Yuetong Wang
  • KD Validated
View Article
mouseIF

Front Neurosci

Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti

Authors - Laura A Lindner
View Article