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  • KD/KO Validated

TUB Polyclonal antibody

TUB Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 17928-1-AP

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Synonyms

rd5, TUB, tubby homolog (mouse), Tubby protein homolog



经过测试的应用

Positive WB detected inmouse brain tissue, mouse uterus tissue, Neuro-2a cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17928-1-AP targets TUB in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IF, ChIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen TUB fusion protein Ag12354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubby homolog (mouse)
Synonyms rd5, TUB, tubby homolog (mouse), Tubby protein homolog
Calculated Molecular Weight 561 aa, 62 kDa
Observed Molecular Weight 56 kDa, 62-67 kDa
GenBank Accession NumberBC075032
Gene Symbol TUB
Gene ID (NCBI) 7275
RRIDAB_2212236
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50607
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for TUB antibody 17928-1-APDownload protocol
IHC protocol for TUB antibody 17928-1-APDownload protocol
IP protocol for TUB antibody 17928-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cells

Differential Roles of Tubby Family Proteins in Ciliary Formation and Trafficking.

Authors - Julie J Hong
humanChIP,WB

Cell Rep

NAD+ supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells.

Authors - Yuetong Wang
  • KD Validated
mouseIF

Front Neurosci

Distribution of ciliary adaptor proteins tubby and TULP3 in the organ of Corti

Authors - Laura A Lindner