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验证数据展示

  • WB analysis using 16007-1-AP

    WB analysis using 16007-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat eye using 16007-1-AP

    IHC staining of rat eye using 16007-1-AP

    Immunohistochemical analysis of paraffin-embedded rat eye tissue slide using 16007-1-AP (Alpha Tubulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Alpha Tubulin Polyclonal antibody

Alpha Tubulin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16007-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, zebrafish, rat

应用

WB, IHC, ELISA

别名

alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, zebrafish tissues, mouse ovary tissues
Positive IHC detected inrat eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16007-1-AP targets Alpha Tubulin in WB, IHC, ELISA applications and shows reactivity with human, mouse, zebrafish, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, zebrafish, rat
免疫原 Alpha Tubulin fusion protein Ag8695 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin, alpha 1c
别名 alpha tubulin, Alpha tubulin 6, TUBA1C, TUBA6, Tubulin alpha 1C chain, Tubulin alpha 6 chain, tubulin, alpha 1c
计算分子量 50 kDa
观测分子量50 kDa
GenBank蛋白编号BC004949
基因名称 Alpha Tubulin
Gene ID (NCBI) 84790
RRIDAB_2303957
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BQE3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
IHC protocol for Alpha Tubulin antibody 16007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols