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TUBD1 Polyclonal antibody

TUBD1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27433-1-AP

Print datasheet

Synonyms

Delta tubulin, TUBD, TUBD1, Tubulin delta chain, tubulin, delta 1

验证数据展示

  • WB analysis using 27433-1-AP

    WB analysis using 27433-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27433-1-AP (TUBD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27433-1-AP targets TUBD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen TUBD1 fusion protein Ag26626 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, delta 1
Synonyms Delta tubulin, TUBD, TUBD1, Tubulin delta chain, tubulin, delta 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight44 kda
GenBank Accession NumberBC000258
Gene Symbol TUBD1
Gene ID (NCBI) 51174
RRIDAB_3669602
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJT1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBD1, also named as TUBD, Tubulin delta chain, tubulin delta 1. The TUBD1 gene encodes a centrosomal protein of 453-amino acids with a molecular mass of 51 kD containing the canonical GTP-binding domain shared with other tubulins. TUBD1 mRNA is preferentially expressed in the sperm head, but it has been also detected in retina, heart, muscle, thyroid, ovary, and colon, among other tissues. At least 6 isoforms of the human protein are produced by alternative splicing (PMID: 28137601). The molecular weights of these 6 isoforms are 51, 45, 22, 27, 44, and 40 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for TUBD1 antibody 27433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols