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TUBGCP2 Polyclonal antibody

TUBGCP2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25856-1-AP

Print datasheet

Synonyms

GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2

验证数据展示

  • WB analysis using 25856-1-AP

    WB analysis using 25856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25856-1-AP (TUBGCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25856-1-AP

    IHC staining of mouse testis using 25856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 25856-1-AP

    IP experiment of HeLa using 25856-1-AP

    IP result of anti-TUBGCP2 (IP:25856-1-AP, 4ug; Detection:25856-1-AP 1:500) with HeLa cells lysate 3920 ug.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, U2OS cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25856-1-AP targets TUBGCP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen TUBGCP2 fusion protein Ag23066 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tubulin, gamma complex associated protein 2
Synonyms GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2
Calculated Molecular Weight 902 aa, 103 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC093770
Gene Symbol TUBGCP2
Gene ID (NCBI) 10844
RRIDAB_2880271
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BSJ2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790).

实验方案

Product Specific Protocols
WB protocol for TUBGCP2 antibody 25856-1-APDownload protocol
IHC protocol for TUBGCP2 antibody 25856-1-APDownload protocol
IP protocol for TUBGCP2 antibody 25856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cell Proteomics

Interactome analysis reveals a link of the novel ALMS1-CEP70 complex to centrosomal clusters

Authors - Franziska Woerz
View Article