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验证数据展示

  • WB analysis using 25856-1-AP

    WB analysis using 25856-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25856-1-AP (TUBGCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 25856-1-AP

    IHC staining of mouse testis using 25856-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25856-1-AP (TUBGCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 25856-1-AP

    IP experiment of HeLa using 25856-1-AP

    IP result of anti-TUBGCP2 (IP:25856-1-AP, 4ug; Detection:25856-1-AP 1:500) with HeLa cells lysate 3920 ug.

TUBGCP2 Polyclonal antibody

TUBGCP2 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 25856-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA and More (1)

别名

GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, U2OS cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25856-1-AP targets TUBGCP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human
免疫原 TUBGCP2 fusion protein Ag23066 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tubulin, gamma complex associated protein 2
别名 GCP 2, GCP2, h103p, hGCP2, hGrip103, hSpc97, SPBC97, Spc97p, TUBGCP2
计算分子量 902 aa, 103 kDa
观测分子量 103 kDa
GenBank蛋白编号BC093770
基因名称 TUBGCP2
Gene ID (NCBI) 10844
RRIDAB_2880271
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BSJ2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUBGCP2 (tubulin-gamma-complex-associated protein 2) s TUBGCP2 is a core component of the g-TuRC nucleation complex (PMID: 3345861). Pathogenic variants in TUBGCP2 cause an autosomal recessive neurodevelopmental trait consisting of a neuronal migration disord (PMID: 31630790).

实验方案

Product Specific Protocols
WB protocol for TUBGCP2 antibody 25856-1-APDownload protocol
IHC protocol for TUBGCP2 antibody 25856-1-APDownload protocol
IP protocol for TUBGCP2 antibody 25856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Mol Cell Proteomics

Interactome analysis reveals a link of the novel ALMS1-CEP70 complex to centrosomal clusters

Authors - Franziska Woerz
View Article