TUFM Polyclonal antibody

TUFM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 26730-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26730-1-AP targets TUFM in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IF, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, zebrafish
免疫原 TUFM fusion protein Ag24981 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Tu translation elongation factor, mitochondrial
别名 EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4
计算分子量 50 kDa
观测分子量 46-50 kDa
GenBank蛋白编号BC010041
基因名称 TUFM
Gene ID (NCBI) 7284
RRIDAB_2880616
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP49411
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

实验方案

Product Specific Protocols
WB protocol for TUFM antibody 26730-1-APDownload protocol
IHC protocol for TUFM antibody 26730-1-APDownload protocol
IF protocol for TUFM antibody 26730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Cell Biol

Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties

Authors - Dennis K Jeppesen
zebrafishWB

Nucleic Acids Res

Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function.

Authors - Qinghai Zhang
WB

Nucleic Acids Res

Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency.

Authors - Qinghai Zhang
ratWB

Sci Total Environ

Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects

Authors - Dongjie Li
ratWB

J Hazard Mater

ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis

Authors - Zhiyuan Tian
humanWB

Clin Cancer Res

Antitumor Activity of a Mitochondrial-Targeted HSP90 Inhibitor in Gliomas

Authors - Shiyou Wei
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