验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, Jurkat cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:8000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26730-1-AP targets TUFM in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IF, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, zebrafish |
免疫原 | TUFM fusion protein Ag24981 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Tu translation elongation factor, mitochondrial |
别名 | EF-Tu, EFTU, EF TuMT, EF Tu, COXPD4 |
计算分子量 | 50 kDa |
观测分子量 | 46-50 kDa |
GenBank蛋白编号 | BC010041 |
基因名称 | TUFM |
Gene ID (NCBI) | 7284 |
RRID | AB_2880616 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P49411 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TUFM, also named as Elongation factor Tu, mitochondrial, is a 452 amino acid protein, which contains 1 tr-type G (guanine nucleotide-binding) domain and belongs to the TRAFAC class translation factor GTPase superfamily. TUFM localizes in the Mitochondrion and promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
实验方案
Product Specific Protocols | |
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WB protocol for TUFM antibody 26730-1-AP | Download protocol |
IHC protocol for TUFM antibody 26730-1-AP | Download protocol |
IF protocol for TUFM antibody 26730-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol Blebbisomes are large, organelle-rich extracellular vesicles with cell-like properties | ||
Nucleic Acids Res Deletion of Mtu1 (Trmu) in zebrafish revealed the essential role of tRNA modification in mitochondrial biogenesis and hearing function. | ||
Nucleic Acids Res Ablation of Mto1 in zebrafish exhibited hypertrophic cardiomyopathy manifested by mitochondrion RNA maturation deficiency. | ||
Sci Total Environ Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects | ||
J Hazard Mater ZLN005 alleviates PBDE-47 induced impairment of mitochondrial translation and neurotoxicity through PGC-1α/ERRα axis | ||
Clin Cancer Res Antitumor Activity of a Mitochondrial-Targeted HSP90 Inhibitor in Gliomas |